StRac1 plays an important role in potato resistance against Phytophthora infestans via regulating H2O2 production.
Identifieur interne : 000049 ( Main/Exploration ); précédent : 000048; suivant : 000050StRac1 plays an important role in potato resistance against Phytophthora infestans via regulating H2O2 production.
Auteurs : Zhiwei Zhang [République populaire de Chine] ; Xiaoluo Zhang [République populaire de Chine] ; Ren Na [République populaire de Chine] ; Shuqing Yang [République populaire de Chine] ; Zaimin Tian [République populaire de Chine] ; Yan Zhao [République populaire de Chine] ; Jun Zhao [République populaire de Chine]Source :
- Journal of plant physiology [ 1618-1328 ] ; 2020.
Abstract
ROP GTPases (Rho-related GTPases from plant), a unique subgroup of the Rho family in plants, is a group of key regulators of different signaling pathways controlling plant growth and development, cell polarity and differentiation, and plant response against biotic and abiotic stresses. The present study determined the potential regulatory mechanism of potato ROP GTPase (StRac1) against Phytophthora infestans (P. infestans) infection. Protein secondary structure analysis indicated that StRAC1 is a Rho GTPase. The expression level of StRac1 was variable in different tissues of potato, with the highest expression in young leaves of both Shepody and Hutou potato varieties. After challenging with P. infestans, the expression level of StRac1was higher in resistance varieties Zihuabai and Longshu 7 than in susceptible varieties Shepody and Desiree. StRAC1 fusion with GFP subcellularly localized at the plasma membrane (PM) in tobacco epidermal cells. The potato with transient or stable over-expression of CA-StRac1 (constitutively active form of StRac1)exhibited a dramatic enhancement of its resistance against P. infestans infections. The increased resistance level in transgenic potato was accompanied with elevated H2O2 levels. Importantly, silencing StRac1 via virus-induced gene silencing (VIGS) in potato resulted in higher susceptibility to P. infestans infection than in control plants. In summary, our data reveal that StRac1 regulates potato resistance against P. infestans via positively modulating the accumulation of H2O2.
DOI: 10.1016/j.jplph.2020.153249
PubMed: 32829122
Affiliations:
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Electronic address: zzw_217@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia</wicri:regionArea><wicri:noRegion>Inner Mongolia</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiaoluo" sort="Zhang, Xiaoluo" uniqKey="Zhang X" first="Xiaoluo" last="Zhang">Xiaoluo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. 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Electronic address: zhaojun@imau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia</wicri:regionArea><wicri:noRegion>Inner Mongolia</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32829122</idno><idno type="pmid">32829122</idno><idno type="doi">10.1016/j.jplph.2020.153249</idno><idno type="wicri:Area/Main/Corpus">000086</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000086</idno><idno type="wicri:Area/Main/Curation">000086</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000086</idno><idno type="wicri:Area/Main/Exploration">000086</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">StRac1 plays an important role in potato resistance against Phytophthora infestans via regulating H<sub>2</sub>O<sub>2</sub> production.</title><author><name sortKey="Zhang, Zhiwei" sort="Zhang, Zhiwei" uniqKey="Zhang Z" first="Zhiwei" last="Zhang">Zhiwei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: zzw_217@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia</wicri:regionArea><wicri:noRegion>Inner Mongolia</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiaoluo" sort="Zhang, Xiaoluo" uniqKey="Zhang X" first="Xiaoluo" last="Zhang">Xiaoluo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: 839630794@qq.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia</wicri:regionArea><wicri:noRegion>Inner Mongolia</wicri:noRegion></affiliation></author><author><name sortKey="Na, Ren" sort="Na, Ren" uniqKey="Na R" first="Ren" last="Na">Ren Na</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035 China. Electronic address: LNAREN@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang</wicri:regionArea><wicri:noRegion>Shijiazhuang</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Shuqing" sort="Yang, Shuqing" uniqKey="Yang S" first="Shuqing" last="Yang">Shuqing Yang</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: 1030092756@qq.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia</wicri:regionArea><wicri:noRegion>Inner Mongolia</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Zaimin" sort="Tian, Zaimin" uniqKey="Tian Z" first="Zaimin" last="Tian">Zaimin Tian</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: 278941949@qq.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia</wicri:regionArea><wicri:noRegion>Inner Mongolia</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Yan" sort="Zhao, Yan" uniqKey="Zhao Y" first="Yan" last="Zhao">Yan Zhao</name><affiliation wicri:level="3"><nlm:affiliation>Institutes of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101 China. Electronic address: zyzy2020@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institutes of Genetics and Developmental Biology, Chinese Academy of Science, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhao, Jun" sort="Zhao, Jun" uniqKey="Zhao J" first="Jun" last="Zhao">Jun Zhao</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: zhaojun@imau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia</wicri:regionArea><wicri:noRegion>Inner Mongolia</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of plant physiology</title><idno type="eISSN">1618-1328</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">ROP GTPases (Rho-related GTPases from plant), a unique subgroup of the Rho family in plants, is a group of key regulators of different signaling pathways controlling plant growth and development, cell polarity and differentiation, and plant response against biotic and abiotic stresses. The present study determined the potential regulatory mechanism of potato ROP GTPase (StRac1) against Phytophthora infestans (P. infestans) infection. Protein secondary structure analysis indicated that StRAC1 is a Rho GTPase. The expression level of StRac1 was variable in different tissues of potato, with the highest expression in young leaves of both Shepody and Hutou potato varieties. After challenging with P. infestans, the expression level of StRac1was higher in resistance varieties Zihuabai and Longshu 7 than in susceptible varieties Shepody and Desiree. StRAC1 fusion with GFP subcellularly localized at the plasma membrane (PM) in tobacco epidermal cells. The potato with transient or stable over-expression of CA-StRac1 (constitutively active form of StRac1)exhibited a dramatic enhancement of its resistance against P. infestans infections. The increased resistance level in transgenic potato was accompanied with elevated H<sub>2</sub>O<sub>2</sub> levels. Importantly, silencing StRac1 via virus-induced gene silencing (VIGS) in potato resulted in higher susceptibility to P. infestans infection than in control plants. In summary, our data reveal that StRac1 regulates potato resistance against P. infestans via positively modulating the accumulation of H<sub>2</sub>O<sub>2</sub>.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32829122</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-1328</ISSN><JournalIssue CitedMedium="Internet"><Volume>253</Volume><PubDate><Year>2020</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of plant physiology</Title><ISOAbbreviation>J Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>StRac1 plays an important role in potato resistance against Phytophthora infestans via regulating H<sub>2</sub>O<sub>2</sub> production.</ArticleTitle><Pagination><MedlinePgn>153249</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0176-1617(20)30139-5</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jplph.2020.153249</ELocationID><Abstract><AbstractText>ROP GTPases (Rho-related GTPases from plant), a unique subgroup of the Rho family in plants, is a group of key regulators of different signaling pathways controlling plant growth and development, cell polarity and differentiation, and plant response against biotic and abiotic stresses. The present study determined the potential regulatory mechanism of potato ROP GTPase (StRac1) against Phytophthora infestans (P. infestans) infection. Protein secondary structure analysis indicated that StRAC1 is a Rho GTPase. The expression level of StRac1 was variable in different tissues of potato, with the highest expression in young leaves of both Shepody and Hutou potato varieties. After challenging with P. infestans, the expression level of StRac1was higher in resistance varieties Zihuabai and Longshu 7 than in susceptible varieties Shepody and Desiree. StRAC1 fusion with GFP subcellularly localized at the plasma membrane (PM) in tobacco epidermal cells. The potato with transient or stable over-expression of CA-StRac1 (constitutively active form of StRac1)exhibited a dramatic enhancement of its resistance against P. infestans infections. The increased resistance level in transgenic potato was accompanied with elevated H<sub>2</sub>O<sub>2</sub> levels. Importantly, silencing StRac1 via virus-induced gene silencing (VIGS) in potato resulted in higher susceptibility to P. infestans infection than in control plants. In summary, our data reveal that StRac1 regulates potato resistance against P. infestans via positively modulating the accumulation of H<sub>2</sub>O<sub>2</sub>.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zhiwei</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: zzw_217@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xiaoluo</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: 839630794@qq.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Na</LastName><ForeName>Ren</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035 China. Electronic address: LNAREN@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Shuqing</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: 1030092756@qq.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Zaimin</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: 278941949@qq.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institutes of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101 China. Electronic address: zyzy2020@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, 010019 China. Electronic address: zhaojun@imau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Plant Physiol</MedlineTA><NlmUniqueID>9882059</NlmUniqueID><ISSNLinking>0176-1617</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">H(2)O(2)production</Keyword><Keyword MajorTopicYN="N">P. infestans</Keyword><Keyword MajorTopicYN="N">Potato</Keyword><Keyword MajorTopicYN="N">Resistance</Keyword><Keyword MajorTopicYN="N">StRac1</Keyword><Keyword MajorTopicYN="N">VIGS</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>05</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32829122</ArticleId><ArticleId IdType="pii">S0176-1617(20)30139-5</ArticleId><ArticleId IdType="doi">10.1016/j.jplph.2020.153249</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Zhiwei" sort="Zhang, Zhiwei" uniqKey="Zhang Z" first="Zhiwei" last="Zhang">Zhiwei Zhang</name></noRegion><name sortKey="Na, Ren" sort="Na, Ren" uniqKey="Na R" first="Ren" last="Na">Ren Na</name><name sortKey="Tian, Zaimin" sort="Tian, Zaimin" uniqKey="Tian Z" first="Zaimin" last="Tian">Zaimin Tian</name><name sortKey="Yang, Shuqing" sort="Yang, Shuqing" uniqKey="Yang S" first="Shuqing" last="Yang">Shuqing Yang</name><name sortKey="Zhang, Xiaoluo" sort="Zhang, Xiaoluo" uniqKey="Zhang X" first="Xiaoluo" last="Zhang">Xiaoluo Zhang</name><name sortKey="Zhao, Jun" sort="Zhao, Jun" uniqKey="Zhao J" first="Jun" last="Zhao">Jun Zhao</name><name sortKey="Zhao, Yan" sort="Zhao, Yan" uniqKey="Zhao Y" first="Yan" last="Zhao">Yan Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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